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. 2014 Nov 19;290(1):556–567. doi: 10.1074/jbc.M114.614701

FIGURE 2.

FIGURE 2.

CRL4CDT2 substrates are protected in mitosis. A and B, HCT116 cells were grown asynchronously (Async.) or synchronized in prometaphase by release from a thymidine block into 100 nm nocodazole for 10 h (M). Cells were irradiated with 20 J/m2 UV to induce PCNADNA loading (DNA repair synthesis) and harvested 2 h later or left untreated as indicated. Endogenous proteins were detected in whole cell lysates by immunoblotting (asterisk in A denotes a nonspecific background band). C, HCT116 cells were synchronized in prometaphase as in A and released into fresh medium for 2.5 h to proceed to G1. Cells were UV-irradiated and harvested 90 min later, followed by immunoblotting for the indicated proteins.