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The pH dependence for deiodination of I2-Tyr by hIYD. The pH profile of log V (A) and log(V/K) (B) were fit by non-linear least square regression using Equation 1. Data represent an average of three independent measurements in the presence of potassium phosphate (■) and Tris-HCl (○) at the indicated pH values. Error bars represent S.D.
