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The pH dependence of substrate binding to hIYD. The affinity of I2-Tyr for hIYD (KD) is reported as the average of three independent measurements in the presence of potassium phosphate (■) and Tris-HCl (○) at the indicated pH values. The curve was generated from the calculated pKa values and pH according to the sum of Equations 1 and 3. Error bars represent S.D.
