TABLE 2.

Pre-steady-state and isotopic exchange kinetic parameters for interaction of MGL with amino acids

Constantsa	Glycine	l-Alanine	l-Norvaline	l-Cycloserine
k1, m−1 s−1	(1.0 ± 0.2) × 104	(7.0 ± 0.1) × 102	(4.0 ± 1.0) × 104	(3.6 ± 1.2) × 104
k-1, s−1	140 ± 30	0.6 ± 0.1	94.0 ± 6.0	1.6 ± 0.3
k2, s−1	1.5 ± 0.1	(6.8 ± 0.5) × 10−3	(6.6 ± 0.4) × 10−3	(6.5 ± 2.5) × 10−3
k−2, s−1	33.5 ± 1.3	(3.6 ± 0.4) × 10−3	(6.3 ± 0.2) × 10−3	(3.8 ± 0.4) × 10−4
k3, s−1	0.3 ± 0.1
k−3, s−1	0.08 ± 0.04
K1, mm	14	0.9	2.3	0.045
K2	22.3	0.53	1.0	0.58
K3	0.27
Ki, mm	49b	3.4	4.7	0.068 ± 0.007
kexα−H, s−1c	20.2 pro-R	2.71	20.6
	1.4×10−3 pro-S
kexβ−H, s−1c		2.63	11.1

^a K_n = k_−n/k_n.

^b K_i values for glycine, l-alanine, and l-norvaline from Ref. 19.

^c k_ex values for glycine from Ref. 35 and l-alanine and l-norvaline from Ref. 19.