Skip to main content
. 2014 Nov 17;290(1):682–690. doi: 10.1074/jbc.M114.617720

FIGURE 1.

FIGURE 1.

Functional characterization of S-TIM5-dCD. A, sequence alignment of S-TIM5-dCD with structurally characterized dCDs. T4 bact., T4 bacteriophage. Displayed are only sequences of loop 3, including the catalytic motif and the allosteric motif highlighting the distinct tryptophan of S-TIM5-dCD allosteric motif. B, deamination activity of S-TIM5-dCD shown in absorbance (290 nm) as a function of time. Protein incubated with dCMP, dCTP, and Mg2+ (line a); dCMP and Mg2+ (line b); dCTP and Mg2+ (line c); CMP, dCTP, and Mg2+ (line d); and dCMP, dCTP, dTTP, and Mg2+ (line e). The ratio of dCTP to dTTP is 1:1. C, relative dCMP deamination activity of S-TIM5-dCD as a function of pH. The error bars represent triplicates. D, relative dCMP deamination activity of S-TIM5-dCD WT and Trp42 mutants in the presence of dCTP and Mg2+. The error bars represent three samples each of three biological replicates.