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. 2014 Aug 12;1(2):ofu061. doi: 10.1093/ofid/ofu061

Fig. 1.

Fig. 1.

Development of a clade 8-specific mismatch amplification mutation-polymerase chain reaction (PCR) assay. Lane M shows the DNA size markers (Tracklt 1 kb Plus DNA Ladder, Life Technologies) including DNA fragments sized 100, 200, 300, 400, 500, 650, 850, 1000 bp, and so on. The black arrows on the right side of both panels indicate positions of the PCR products. Two different strains from each clade were used. (A) Polymerase chain reaction performed using the 539C-R primer detected strains belonging to clades 1–7, but not strains belonging to clade 8. (B) Polymerase chain reaction performed using the 539A-R primer detected strains belonging to clade 8, but not strains belonging to clades 1–7.