Table 3.
Partial List of Differentially Expressed Proteins in B. subtilis after Plumbagin Treatment Obtained from iTRAQ-Based Quantitative Proteome Analysisa
| Orbitrap | Q-TOF | |||||||
|---|---|---|---|---|---|---|---|---|
| Accession | Name of the protein | Gene name | Coverage | Unique peptides | Plumbagin/control | Coverage | Unique peptides | Plumbagin/control |
| TCA cycle | ||||||||
| P09124 | Glyceraldehyde-3-phosphate dehydrogenase 1 | GapA | 42.39 | 12 | 2.31 | 56.72 | 13 | 2.647 |
| Q03224 | Fructose-1,6-bisphosphatase class 2 | glpX | 22.43 | 6 | 0.464 | – | – | – |
| P54533 | Dihydrolipoyl dehydrogenase | BfmBC | 19.20 | 8 | 0.377 | 15.61 | 5 | 0.520 |
| P80886 | Succinyl-CoA ligase [ADP-forming] subunit beta | SucC | 47.53 | 16 | 0.386 | 62.08 | 22 | 0.495 |
| P80865 | Succinyl-CoA ligase [ADP-forming] subunit alpha | sucD | 29.00 | 5 | 0.465 | – | – | – |
| P08066 | Succinate dehydrogenase iron-sulfur subunit | sdhB | 18.97 | 4 | 0.313 | – | – | – |
| P39126 | Isocitrate dehydrogenase [NADP] OS | Icd | 36.17 | 15 | 0.581 | 55.32 | 21 | 0.488 |
| P09339 | Aconitate hydratase OS | CitB | 23.54 | 17 | 0.317 | 24.31 | 16 | 0.427 |
| P39120 | Citrate synthase 2 OS | CitZ | 27.15 | 7 | 0.454 | 37.90 | 12 | 0.471 |
| Electron transport | ||||||||
| P94551 | Electron transfer flavoprotein subunit alpha | etfA | 9.54 | 2 | 0.478 | – | ||
| P80861 | NADH dehydrogenase-like protein YjlD | yjlD | 30.10 | 8 | 0.335 | 51.53 | 16 | 0.499 |
| O32117 | NADH dehydrogenase-like protein YutJ | yutJ | 12.11 | 3 | 0.552 | – | – | – |
| P42974 | NADH dehydrogenase OS | AhpF | 25.15 | 12 | 1.57 | 35.36 | 13 | 1.592 |
| P54524 | Probable NADH-dependent flavin oxidoreductase YqiG OS | yqiG | 18.82 | 5 | 3.926 | 24.73 | 6 | 1.240 |
| P42175 | Nitrate reductase alpha chain | narG | 4.72 | 4 | 1.650 | – | – | – |
| P42176 | Nitrate reductase beta chain | narH | 5.95 | 2 | 2.469 | – | – | – |
| P39605 | FMN reductase (NADPH) | nfrA1 | 24.10 | 4 | 2.774 | 34.54 | 5 | 1.262 |
| P94424 | FMN reductase [NAD(P)H] | nfrA2 | 20.88 | 4 | 7.150 | 16.06 | 3 | 6.020 |
| Fatty acid biosynthesis | ||||||||
| P49786 | Biotin carboxyl carrier protein of acetyl-CoA carboxylase OS | accB | 28.93 | 2 | 0.464 | 33.33 | 3 | 0.698 |
| P49787 | Biotin carboxylase 1 OS | accC1 | 18.22 | 6 | 0.462 | 26.44 | 8 | 0.707 |
| P94549 | Probable enoyl-CoA hydratase OS | fadB | 10.85 | 2 | 0.581 | |||
| P94584 | 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ OS | fabZ | 9.93 | 2 | 1.799 | 5.67 | 1 | 3.105 |
| O07600 | 3-oxoacyl-[acyl-carrier-protein] synthase 3 protein 2 OS | fabHB | 7.08 | 2 | 0.634 | – | – | – |
| P54616 | Enoyl-[acyl-carrier-protein] reductase [NADH] FabI OS | fabI | 39.53 | 9 | 0.440 | 27.91 | 6 | 0.595 |
| Cell wall and cell division | ||||||||
| P28264 | Cell division protein FtsA OS | FtsA | 11.36 | 4 | 0.529 | |||
| P28015 | Putative septation protein SpoVG OS | SpoVG | 40.21 | 3 | 0.475 | |||
| P26497 | Stage 0 sporulation protein J OS | Spo0J | 32.27 | 7 | 0.563 | |||
| P45693 | Stage V sporulation protein S OS | SpoVS | 38.37 | 2 | 0.617 | |||
| Q02114 | N-acetylmuramoyl-L-alanine amidase LytC | lytC | 5.24 | 2 | 0.221 | – | – | – |
| P54421 | Probable peptidoglycan endopeptidase LytE | lytE | 8.08 | 2 | 0.266 | – | – | – |
| P96612 | D-alanine-D-alanine ligase OS | ddl | 5.93 | 2 | 0.644 | – | – | – |
| P80698 | Trigger factor OS | Tig | 36.08 | 13 | 3.354 | 59.67 | 20 | 1.664 |
| P19670 | UDP-N-acetylglucosamine 1-carboxyvinyltransferase 2 OS | MurAB | 11.66 | 3 | 2.115 | 10.96 | 3 | 2.120 |
| P39131 | UDP-N-acetylglucosamine 2-epimerase | MnaA | 11.32 | 3 | 1.639 | – | – | – |
| P42976 | 4-hydroxy-tetrahydrodipicolinate reductase | dapB | 14.98 | 2 | 1.799 | 40.07 | 7 | 2.647 |
| Heme biosynthesis | ||||||||
| P30950 | Delta-aminolevulinic acid dehydratase | HemB | 34.26 | 7 | 2.42 | 64.20 | 15 | 1.944 |
| P32396 | Ferrochelatase OS | HemH | 22.58 | 6 | 2.09 | 26.13 | 6 | 1.369 |
| P30949 | Glutamate-1-semialdehyde 2,1-aminomutase | HemL | 21.86 | 6 | 2.06 | 34.88 | 9 | 2.022 |
| P16616 | Porphobilinogen deaminase OS | HemC | 27.07 | 6 | 1.86 | 35.03 | 8 | 2.116 |
| Protein synthesis | ||||||||
| P21473 | 30S ribosomal protein S15 OS | rpsO | 19.10 | 4 | 4.689 | 41.57 | 3 | 1.300 |
| P21474 | 30S ribosomal protein S16 OS | rpsP | 55.56 | 4 | 3.141 | 63.33 | 5 | 2.220 |
| P21476 | 30S ribosomal protein S19 OS | rpsS | 50.00 | 6 | 3.357 | 48.91 | 5 | 1.735 |
| P21477 | 30S ribosomal protein S20 OS | rpsT | 23.86 | 2 | 15.518 | 26.14 | 3 | 1.220 |
| P21470 | 30S ribosomal protein S9 OS | rpsI | 49.23 | 4 | 2.190 | 49.23 | 4 | 1.698 |
| Q06796 | 50S ribosomal protein L11 OS | rplK | 44.68 | 6 | 1.814 | 66.67 | 8 | 1.430 |
| P19946 | 50S ribosomal protein L15 OS | rplO | 40.41 | 5 | 1.908 | 52.05 | 7 | 1.684 |
| P20277 | 50S ribosomal protein L17 OS | rplQ | 45.00 | 5 | 1.975 | 39.17 | 5 | 1.273 |
| P05657 | 50S ribosomal protein L27 OS | rpmA | 43.62 | 5 | 6.451 | 43.62 | 5 | 3.351 |
| P42920 | 50S ribosomal protein L3 OS | rplC | 39.23 | 6 | 1.599 | 48.33 | 7 | 2.472 |
| P19947 | 50S ribosomal protein L30 OS | rpmD | 52.54 | 4 | 6.322 | 42.37 | 4 | 1.251 |
| O34967 | 50S ribosomal protein L31 type B OS | rpmE2 | 30.49 | 2 | 2.998 | – | – | – |
| P55874 | 50S ribosomal protein L35 OS | rpmI | – | – | – | 45.45 | 4 | 0.434 |
a
Full list of the proteins are described in Supplementary Table ST2.