TABLE 1 .
Descriptive statistics of cell sizea
| Strain | Zn2+ | Min | First quartile |
Median | Third quartile |
Max | Mean | SD | Width | Asymmetric division (%) |
Minicells (%) |
Anucleate cells (%) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sp1 | − | 1.00 | 1.53 | 1.77 | 2.08 | 2.81 | 1.83 | 0.39 | 0.67 | 0.00 | 0.00 | 0 |
| Sp57 | − | 0.66 | 1.34 | 1.59 | 1.88 | 2.66 | 1.61 | 0.39 | 0.66 | 31.80 | 5.50 | 2.5 |
| Sp60 | − | 0.63 | 1.29 | 1.55 | 1.83 | 3.01 | 1.57 | 0.44 | 0.65 | 29.00 | 10.25 | 2.7 |
| Sp60 | + | 1.04 | 1.47 | 1.67 | 1.97 | 2.78 | 1.75 | 0.36 | 0.67 | 0.75 | 0.00 | 0 |
a
Cell size (µm) of 400 cells in each strain was measured using automated MicrobeTracker software, and cell length results were analyzed by GraphPad Prism 3.0 (columns 3 to 10). Min, minimum; Max, maximum; SD, standard deviation; Zn2+, strain was cultivated in the presence of 0.45 mM ZnCl2 to induce expression of His6-LocZ. The number of asymmetrically dividing cells was evaluated from phase-contrast microscopic pictures. Minicells represent all cells shorter than 1 µm (the minimal length of WT cells).