Figure 2. Early β-cell apoptosis is potentiated by overexpression of miR-21, but reduced by knockdown of miR-34a both in the absence and the presence of cytokines. Cells (50 000 cells) were left untreated (A) or were exposed to IL-1β and IFN-γ for 24 h (B). Nucleosomal fragments in adherent cell lysates were detected by ELISA (Cell Death Detection ELISA). Results of 10 independent experiments are shown as means + SEM. Statistical significance was determined using a 2-way paired t test. *** P < 0.001 vs. miR-neg.