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. 2015 Jan 1;128(1):140–149. doi: 10.1242/jcs.161588

Fig. 4.

Fig. 4.

E-cadherin reinforces the nectin junctions. Parental A431D cells (A431D) and their derivatives expressing EcadDn (EcDn) or cis-EcadDn (cis-EcDn) were incubated for 20 min with BC-12 antibody (10 µg/ml) at 37°C (37°C) and then double-stained using anti-mouse antibody (Nt), detecting localization of BC-12–nectin-2 complexes, and rabbit anti-Dendra antibody (Dn), detecting recombinant Dendra-tagged E-cadherin. The control cells (4°C) were incubated with BC-12 antibody (20 min, 10 µg/ml) on ice. Higher magnifications of the selected regions (indicated by arrows) are shown in the insets. Scale bars: 10 µm. Note that BC-12 antibody disintegrates nectin junctions in A431D cells but is unable to do so in presence of cadherin.