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Volumetric GalOx activity in the fermentation broth at 25 °C (▪) and 30 °C (♦). Time corresponds to the total time of cultivation. Methanol feeding was initiated at 30 h. The detectable production of active and correctly folded GalOx sets off earlier at 30 °C, apparently due to the higher biomass concentration (data not shown). It stabilizes after 85 h of culture. At 25 °C, the volumetric GalOx activity increases continuously to 600 kU L−1.
