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. 2014 Feb 16;44(4):962–969. doi: 10.1002/eji.201344065

Figure 4.

Figure 4

Salmonella and curdlan-induced peptide presentation to type B (11A10) T cells is blocked by anti-Dectin-1 Ab and laminarin. (A) BMDCs were either directly exposed to HKST or preincubated for 1 h with anti-Dectin-1 (2A11) Ab (25 μg/mL), laminarin (500 μg/mL), or anti-Dectin-1 Ab 2A11 (25 μg/mL) and laminarin (500 μg/mL) combined. After 24 h, presentation of HEL46–61 peptide to type B hybridoma (11A10) cells was measured by IL-2 production as described in Figure1. Data are shown as mean ± SEM of 12 samples pooled from four independent experiments. (B) BMDCs were treated as in (A), but exposed to curdlan (100 μg/mL) in place of HKST. Data are shown as mean ± SEM of nine samples pooled from four independent experiments. (C) Comparison of the efficiency of combined laminarin and anti-Dectin Ab blocking upon curdlan and HKST induced peptide presentation to type B T cells plotted as paired datasets. p-Values were calculated using Student's paired t-test.