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. 2014 Sep 2;112(1):141–155. doi: 10.1002/bit.25332

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© 2014 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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G0/G1 arrest improves glycan processing of mAb. The indicated recombinant CHO cell lines expressing different monoclonal antibodies were subjected to 5-day plate-based production culture with or without CCI treatment. Day 5 production supernatants were collected and protein A-purified and the glycan profiles of the mAbs were analyzed using HILIC method to determine the structures and relative levels of different glycan structures. Average percentage of High mannose (HM) (A) and G1F + G2F (B) from the indicated cell lines are shown. Data represent the average ± SD of triplicate samples. Details of glycan profile and structures of the N-linked glycan species detected on mAb from cell line C was analyzed by HILIC assay (C). *P < 0.05, **P < 0.01, and ***P < 0.001 represent statistically significant differences between untreated controls and different CCI treated conditions.