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. 2014 Sep 2;112(1):141–155. doi: 10.1002/bit.25332

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© 2014 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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The comparison of different growth control approaches in bioreactors. A recombinant CHO cell line was cultured in production bioreactors as described in the Materials and Methods section. 10 µM of inhibitor was added to the indicated bioreactor at day 8. For the temperature shift condition, the culture temperature was decreased from 36 to 30°C at day 8. For the Low Asn condition, perfusion medium with 5 mM Asn was substituted for the standard 17.3 mM for the indicated bioreactor. VCD (A) and viability (B) were measured daily. Production supernatants were collected and protein A-purified. qP (C), titer (D), HMW (E), high mannose (HM) (F), and terminal galactose (G) were analyzed as described in the Materials and Methods section. Each point represents the average and standard deviation of duplicate measurements from two independent bioreactor runs.