Table 2.
List of plasmids used in this study
| Name | Description | Reference |
|---|---|---|
| Integrative plasmid set with URA3 selection marker flanked with direct repeats | ||
| pCfB126 | pX-2-USER-URA3-DR | Mikkelsen et al. (2012) |
| pCfB127 | pX-3-USER-URA3-DR | Mikkelsen et al. (2012) |
| pCfB128 | pX-4-USER-URA3-DR | Mikkelsen et al. (2012) |
| pCfB383 | pXI-1-USER-URA3-DR | Mikkelsen et al. (2012) |
| pCfB384 | pXI-2-USER-URA3-DR | Mikkelsen et al. (2012) |
| pCfB385 | pXI-3-USER-URA3-DR | Mikkelsen et al. (2012) |
| pCfB387 | pXI-5-USER-URA3-DR | Mikkelsen et al. (2012) |
| pCfB129 | pXII-1-USER-URA3-DR | Mikkelsen et al. (2012) |
| pCfB120 | pXII-2-USER-URA3-DR | Mikkelsen et al. (2012) |
| pCfB130 | pXII-4-USER-URA3-DR | Mikkelsen et al. (2012) |
| pCfB131 | pXII-5-USER-URA3-DR | Mikkelsen et al. (2012) |
| pCfB49 | pXII-1-ccdB-USER-URA3-DR | Mikkelsen et al. (2012) |
| Plasmids that contain LoxP-flanked selection marker cassettes | ||
| pUG6 | LoxP-KanMX | Gueldener et al. (2002) |
| pUG27 | LoxP-SpHIS5 | Gueldener et al. (2002) |
| pUG72 | LoxP-KlURA3 | Gueldener et al. (2002) |
| pUG73 | LoxP-KlLEU2 | Gueldener et al. (2002) |
| pSA40 | LoxP-CaLYS5 | Ito-Harashima & McCusker (2004) |
| Episomal replication vectors with USER cassette (the vectors were derived from pESC vector series, Agilent) | ||
| pCfB54 | pESC-URA-ccdB-USER | This study |
| pCfB55 | pESC-HIS-ccdB-USER | This study |
| pCfB132 | pESC-URA-USER | This study |
| pCfB291 | pESC-HIS-USER | This study |
| pCfB220 | pESC-LEU-USER | This study |
| EasyClone integrative vector set with loxP-flanked selection markers | ||
| pCfB255 | pX-2-LoxP-KlURA3 | This study |
| pCfB353 | pX-2-LoxP-KanMX | This study |
| pCfB257 | pX-3-LoxP-KlLEU2 | This study |
| pCfB258 | pX-4-LoxP-SpHIS5 | This study |
| pCfB388 | pXI-1-LoxP-KlLEU2 | This study |
| pCfB389 | pXI-2-LoxP-KlURA3 | This study |
| pCfB390 | pXI-3-LoxP-KlURA3 | This study |
| pCfB391 | pXI-5-LoxP-SpHIS5 | This study |
| pCfB259 | pXII-1-LoxP-KlLEU2 | This study |
| pCfB260 | pXII-2-LoxP-CaLYS5 | This study |
| pCfB262 | pXII-4-LoxP-SpHIS5 | This study |
| pCfB261 | pXII-5-LoxP-SpHIS5 | This study |
| Plasmids containing genes encoding fluorescent proteins | ||
| pWJ1163 | CFP | Reid et al. (2002) |
| pWJ1165 | YFP | Reid et al. (2002) |
| pWJ1350 | RFP | Lisby et al. (2003) |
| EasyClone vectors for expression of genes for fluorescent proteins in S. cerevisiae | ||
| pCfB393 | pX-2-LoxP-KlURA3-TEF1::CFP | This study |
| pCfB394 | pX-3-LoxP-KlLEU2-TEF1::RFP | This study |
| pCfB395 | pX-4- LoxP-SpHiS5-TEF1::YFP | This study |
| Episomal vectors for expression of genes for fluorescent proteins in S. cerevisiae | ||
| pCfB396 | pESC-URA-USER-TEF1::CFP | This study |
| pCfB397 | pESC-LEU-USER-TEF1::RFP | This study |
| pCfB398 | pESC-HIS-USER-TEF1::YFP | This study |
| Plasmid contains a cassette for expression of GFP in E. coli (used as positive control in USER cloning) | ||
| pCfB774 | pmExpCtrl | Dr Hao Lao, DTU |