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. Author manuscript; available in PMC: 2015 May 24.
Published in final edited form as: Dev Cell. 2014 Nov 24;31(4):434–447. doi: 10.1016/j.devcel.2014.10.015

Figure 5. Eya1 Interacts with Six2 and Myc and Regulates Myc Postphosphorylation Modification in Nephron Progenitors.

Figure 5

(A) CoIP analysis. Antibodies for IP and western blot are indicated.

(B–D) Immunostaining of E13.5 kidney sections for pT58 (B), C-Myc (C), and N-Myc/pHH3 (D).

(E) Western blot of cell extracts from E12.5 wild-type or CKO kidneys (Tm at E11.0) with the indicated antibodies. The membrane was stripped and reprobed.

(F–J′) Immunostaining of E12.5 kidney sections for pT58 (Fand F′), N-Myc/pT58(G and G′), C-Myc/pT58 (Hand H′), PCNA/γH2AX (I and I′), and p27Kip1 (J and J′) in the wild-type (F–J) and mutant (F′–J′).

See also Figure S5.