(A) CoIP analysis. Antibodies for IP and western blot are indicated.
(B–D) Immunostaining of E13.5 kidney sections for pT58 (B), C-Myc (C), and N-Myc/pHH3 (D).
(E) Western blot of cell extracts from E12.5 wild-type or CKO kidneys (Tm at E11.0) with the indicated antibodies. The membrane was stripped and reprobed.
(F–J′) Immunostaining of E12.5 kidney sections for pT58 (Fand F′), N-Myc/pT58(G and G′), C-Myc/pT58 (Hand H′), PCNA/γH2AX (I and I′), and p27Kip1 (J and J′) in the wild-type (F–J) and mutant (F′–J′).
See also Figure S5.