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. Author manuscript; available in PMC: 2015 May 24.
Published in final edited form as: Dev Cell. 2014 Nov 24;31(4):434–447. doi: 10.1016/j.devcel.2014.10.015

Figure 8. Eya1 Targets the T58 Phosphate of Myc.

Figure 8

(A) In vitro phosphatase assay. Purified Myc protein was phosphorylated by GSK3β with 32P-γATP. The phosphorylated proteins were incubated with FLAG-Eya1, analyzed on SDS-PAGE, and exposed by phosphorimager.

(B) Eya1 dephosphorylates Myc at T58 but not S62. Purified Myc was incubated with either buffer (−) or FLAG-Eya1 at 30°C for 15 or 30 min. Samples were run in triplicate and immunoblotted with anti-pT58, anti-pS62, or anti-FLAG as indicated.

See also Figure S8.a