Table ED2.

Growth of mutant B. ovatus strains on xyloglucan relative to wild-type B. ovatus 8384.

Strain		Relative Growth* (%)	Relative Rate (ΔA600/Δtime)	Relative Lag (ΔtimeMutant/ΔtimeWT)
ΔGH5	tamarind xyloglucan	No Growth
	dp14 tamarind xyloglucan	139 ± 17†	2.2 ± 0.2†	1.2 ± 0.1
	GH5-digested tamarind xyloglucan	70 ± 9	0.6 ± 0.2‡	1.2 ± 0.2
	XGOs (Megazyme)	71 ± 6‡	0.6 ± 0.1†	1.3 ± 0.3
ΔGH9	tamarind xyloglucan	119 ± 11	1.2 ± 0.2	1.0 ± 0.1
	dp14 tamarind xyloglucan	114 ± 18	0.9 ± 0.1	1.1 ± 0.1
	GH5-digested tamarind xyloglucan	105 ± 6	1.0 ± 0.1	1.3 ± 0.3
	XGOs (Megazyme)	95 ± 13	0.9 ± 0.1	1.2 ± 0.2
ΔGH31	tamarind xyloglucan	18 ± 2†	0.06 ± 0.05†	3.3 ± 0.8†
	dp14 tamarind xyloglucan	No Growth
	GH5-digested tamarind xyloglucan	No Growth
	XGOs (Megazyme)	No Growth

^*Growth conditions are described in Methods. Relative growth values are average percent of wild-type B. ovatus 8384 growth density. No growth was defined as an increase of OD₆₀₀ < 0.05, measured from the initial baseline to the maximum achieved. Standard errors of mean values for three biological replicates are indicated.

^†p value ≤0.05 as determined by unpaired t-test

^‡p value = 0.05–0.1 as determined by unpaired t-test