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Relative Aqp5 mRNA levels in preimplantation stage embryos in vivo. Real-time RT-PCR was used to determine the relative mRNA levels of Aqp5 in unfertilized oocyte (UF), pronucleus stage unfertilized egg (PN), 2-cell (2-C), 4-cell (4-C), 8-cell (8-C), morula (Mor), and blastocyst (Bla) which were developed in vivo. Ten embryos were used for real-time RT-PCR per embryonic stage and repeated 3 times in all stages. * Significant difference among stages (p<0.05, ANOVA).
