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Cloning of mCARM1 in modified pET-20b vector and characterization of the recombinant CPP-CARM1 protein. A. Sequence analysis of mouse CARM1 which cloned in pET20b. Mouse CARM1 was connected with seven arginine at 5' end and six histidine at 3' end. B. Confirmation of pure isolation of CPP-CARM1 by Coomasie blue staining and Western blot using specific antibody against 6x His-taq.
