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Sequences of primers used to extend mel 1a partial sequence and RACE PCR
| Sequence | ||
|---|---|---|
| Extension of mel 1a | Forward | 5'-ACG CAG GGA ACA TCT TTG TG-3' |
| Reverse | 5'-TGA CCT GGT TGT TGT TGG TC-3' | |
| 3' RACE | Forward 1 | 5'-GCT GAC GCC ACA TGA CGT TA-3' |
| Forward 2 | 5'-TTC ATC GGA CTG GCT GTA GC-3' | |
| 5' RACE | Reverse 1 | 5'-ATC GTG CCC AAC CTG TTT GT-3' |
| Reverse 2 | 5'-TTC CTC ATG GGT GTC AGC GT-3' | |
| Reverse 3 | 5'-GCA GTA GCA GAC CTT GTG GT-3' | |
