Table 2.
Sequences of primers used for expression studies
| Sequence | Product size (bp) | ||
|---|---|---|---|
| Tissue distribution (RT-PCR) | mel 1a F | 5'-ACG CAG GGA ACA TCT TTG TG-3' | 318 |
| mel 1a R | 5'-CAC AAA CAG GTT GGG CAC GAT-3' | ||
| mel 1b F | 5'-TCG CCC AGA ATG TCA GCA GT-3' | 221 | |
| mel 1b R | 5'-GGC CCA GCA AAT GGC AAA CA-3' | ||
| mel 1c F | 5'-TAC CCC TAC CCA CTG GTC CT-3' | 234 | |
| mel 1c R | 5'-TGG CGG TGA GTA GCC AAG TA-3' | ||
| β-actin F | 5'-AAT CGT GCG TGA CAT CAA GG-3' | 392 | |
| β-actin R | 5'-AGT ATT TAC GCT CAG GTG GG-3' | ||
| Quantitative real-time PCR | qmel 1a F | 5'-GGT TGT GAT TCC CCT CAT CCC-3' | 105 |
| qmel 1a R | 5'-TTC TGG TTC AGC ACG CCA TAC A-3' | ||
| GnRHr I F * | 5'-GTG GCT TGC CGG AGA CTT TG-3' | 123 | |
| GnRHr I R * | 5'-AGA GGG TTG AGG ATG GCT GAC T-3' | ||
| qβ-actin F | 5'-AGC ATC CCG TCC TGC TCA CA-3' | 121 | |
| qβ-actin R | 5'-AGC ACA GCC TGG ATG GCA AC-3' | ||
*
GnRHr I gene primer from Martinez-Chavez et al. (2008). Primers for mel 1a, mel 1b (GenBank accession No. XM003446809.1), mel 1c (Kim et al., unpublished) and β-actin (GenBank accession No. XM003443127.1) genes were newly designed.