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Transactivation of reporter genes through the LBE. The pCMV Tag5 or pCMV Tag5-Lhx8 was co-transfected with 3xLBE-pGL4 luciferase reporter vector into HEK293 cells. Cell extracts were collected after 48h of transfection and analyzed for luciferase activity. The black box indicates the artificial 3xLBE (TGATTG). (* P<0.01)
