Fig. 1.

Transgenic constructs, characterization of the genomic insertion and expression of transgenes. (a) Schematic representation of the transgenic constructs. The Flag-tagged transgenes (Flag-hCenexin^WT, Flag-hCenexin1^S796A and Flag-hODF2) are under control of the CMV-1E enhancer and chicken β-actin promoter. (b) Southern blot analysis showing the presence of transgenes in mouse genomic DNA. The genomic DNA was digested with BamH1 and probed with a ³²P-labeled. (c) Genotypes of the endogenous mODF2 were determined with genomic PCR. The 676 and 147 bp bands represent wild type and deletion mutation of the endogenous mODF2 gene, respectively. The transgenes are not amplified with the primers used in this PCR analysis. (d) Tail extracts of the wild type and hODF2/Cenexin1 transgenic mice were subjected to immunoblot analysis with the hCenexin1 antibody. Arrow heads indicate specific signals of the transgene proteins. Asterisks represent non-specific cross-reacting bands.