Fig. 3.

Fate of M. agalactiae after entry into the eukaryotic cells. HeLa-229 cells were incubated with M. agalactiae type strain PG2 for 24 h followed by gentamicin treatment for 3 h at 37 °C. Cells were washed and incubated with fresh MEM with (black squares) or without (black circles) gentamicin for additional 8, 16 and 24 h, and then trypsinized and plated to enumerate the viable mycoplasmas. Mean values ± SD from three independent experiments performed in duplicate are indicated.