Table 1.
Qualitative bacteriological examination of lymph nodes and organs from sheep inoculated by the intramammary route with 109 viable cfu of M. agalactiae type strain (PG2) and necropsied at Day 16 p.i.
| Organs/Tissue samples | PCRa | Cultureb | Immunohistochemistryc |
|---|---|---|---|
| Supramammary LNd right | + | + | + |
| Parotid LN right | + | + | NDe |
| Parotid LN left | + | + | ND |
| Popliteal LN right | + | + | + |
| Popliteal LN left | + | + | + |
| Liver | + | + | − |
| Udder right | + | + | + |
| Udder left | + | + | − |
| Lung left | + | + | + |
| Lung right | + | + | + |
| Kidney right | + | + | ND |
| Spleen | − | − | + |
| Heart | + | + | − |
| Uterus (fallopian tube) right | + | + | − |
| Uterus left | + | + | − |
| Capsule (carpal joint) left | + | + | ND |
| Capsule right | + | + | ND |
| Brain | + | + | + |
| Synovial fluid (knee joint) right | + | + | ND |
a
M. agalactiae 16S rRNA specific PCR results.
b
M. agalactiae colonies obtained on Aluotto agar by plating the broth subcultures of various tissue and lymph node (LN) samples.
c
IHC: immunohistochemical analysis of selected M. agalactiae cultures.
d
LN: lymph node.
e
ND: not done.