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Characterization of purified Eht1. (a) Size-exclusion chromatogram of detergent-solubilized Eht1 consistent with an apparent homopentamer, relative to protein standards as shown. Fractions were collected and applied to an SDS–PAGE gel to confirm the peak identity as Eht1 and to verify that that no other proteins were co-purified. (b) Eht1 exhibits multiple bands on blue native PAGE
