Table 1.
Bacterial strains and plasmids used in this study
| Genotype or characteristics | Source or reference | |
|---|---|---|
| Strains | ||
| L. acidophilus | ||
| NCFM | Human intestinal isolate | Barefoot and Klaenhammer (1983) |
| NCK1909 | NCFM carrying a 315 bp in-frame deletion within the upp gene; parent/background strain for all deletion mutants | Goh et al. (2009) |
| NCK1910 | NCK1909 harbouring pTRK669; host for pORI-based counterselective integration vector | Goh et al. (2009) |
| NCK2180 | NCK1909 carrying a 1347 bp in-frame deletion within glgA (LBA0683) | This study |
| NCK2277 | NCK1909 carrying a 1779 bp in-frame deletion within glgB (LBA0680) | This study |
| NCK2182 | NCK1909 carrying a 2280 bp in-frame deletion within glgP (LBA0685) | This study |
| NCK2280 | NCK1909 carrying a 1674 bp in-frame deletion within amy (LBA0686) | This study |
| E. coli | ||
| EC101 | RepA+ JM101; kanamycin-resistant; repA from pWV01 integrated in chromosome; cloning host for pORI-based plasmids | Law et al. (1995) |
| Plasmids | ||
| pTRK935 | 3.0 kb; pORI-based counterselective integration vector containing a native L. acidophilus NCFM upp expression cassette and lacZ' multiple cloning sites from pUC19 | Goh et al. (2009) |
| pTRK1042 | 4.6 kb; pTRK935 with a mutated copy of glgA cloned into BamHI/SacI sites | This study |
| pTRK1071 | 4.3 kb; pTRK935 with a mutated copy of glgB cloned into HindIII/EcoRI sites | This study |
| pTRK1043 | 4.3 kb; pTRK935 with a mutated copy of glgP cloned into BamHI/SacI sites | This study |
| pTRK1072 | 4.3 kb; pTRK935 with a mutated copy of amy cloned into BamHI/SacI sites | This study |