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. 2014 Sep 5;33(21):2521–2533. doi: 10.15252/embj.201488663

Figure 6. Role of FANCJ in promoting G-quadruplex structure replication is independent of FANCD2.

Figure 6

  1. G4G3N and non-G4C3p plasmids were replicated in HSS. Samples were taken at various time points and analyzed by Western blotting using xlFANCD2 antibody.
  2. Mock-depleted and FANCD2-depleted HSS were analyzed by Western blotting using xlFANCJ and xlFANCD2 antibodies. A dilution series of undepleted extract was loaded on the same blot to determine the degree of depletion. A relative volume of 100 corresponds to 0.7 μl of HSS. A non-specific band cross-reacting with FANCJ antibody is used as loading control (‘Loading’).
  3. Mock-depleted and FANCD2-depleted HSS from (B) were used to replicate the G4G3N template starting from primer A. Replication products were extracted, separated on 6% urea-PAGE gels, and visualized by autoradiography.