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. 2014 Sep 26;33(21):2581–2596. doi: 10.15252/embj.201488351

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© 2014 The Authors. Published under the terms of the CC BY 4.0 license

This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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A Schematic illustrating the protein array screen for MEKK1 PHD substrates using UBE1, UBE2N:UBE2V1 and MEKK1 PHD in a ubiquitination assay.

B Ubiquitination assays were performed comprising UBE1, UBE2N:UBE2V1 and MEKK1 PHD or UBE1 and UBE2N:UBE2V1 in the presence of biotin-Ub and profiled using Protoarray Human Protein Microarrays v.5.

C A heat map comparing hits between UBE1 + UBE2N:UBE2V1 and UBE1 + UBE2N:UBE2V1 + MEKK1 PHD reactions.

D–H Ubiquitination of (D) TAB1, (E) TNIP1, (F) TNIP2, (G) TRAF2, and (H) STAM1 by MEKK1. HEK 293 cells were transfected as indicated, lysates prepared and analysed by IP and IB with the indicated antibodies.

Data information: Results are representative of three independent experiments.

Source data are available online for this figure.