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. 2014 May 10;29(4):156–173. doi: 10.1111/omi.12052

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© 2014 The Authors. Molecular Oral Microbiology published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Absorption experiment using unlabeled Arg-hgp and Lys-hgp. The enzyme-labeled antigen method for Arg-hgp reactivity and Lys-hgp reactivity on consecutive frozen sections in case 1 is illustrated. The reactivities to Arg-hgp and Lys-hgp in the cytoplasm of plasma cells (left panels) are abolished with an excess amount of the corresponding unlabeled proteins (center top and right bottom panels), and partly eliminated with unlabeled Lys-hgp and Arg-hgp, respectively (center bottom and right top panels). Arrowheads indicate plasma cells producing antibodies reactive to the unique epitope on Arg-hgp or Lys-hgp. Bar indicates 50 μm.