Table 4.

Summary of (i) IgG concentration in the serum and tissue extract, (ii) plasma cell density in the subepithelial layer of the biopsied gingival tissue, (iii) percentage of plasma cells producing antibodies reactive to Porphyromonas gingivalis, and (iv) the ΔΔC_t value, representing a relatively quantitative value, for the P. gingivalis 16S ribosomal RNA gene

Case	IgG concentration (mg ml−1)		Plasma cell density (cells mm−2)	Percentage of specific plasma cells in tissue1	Real-time PCR for P. gingivalis genome2
	Serum	Tissue extract
1	9.3	0.25	392	10.4	1.00
2	10.8	0.19	307	22.3	0.46
3	17.7	0.32	168	2.8	13.93
4	10.8	0.19	164	1.3	0.01
5	9.0	0.27	132	1.5	112.21
6	16.2	0.17	132	10.0	709.18
7	12.9	1.10	130	6.8	–
8	7.8	0.14	128	0.9	194.01
9	15.0	0.38	127	4.2	1.96
10	18.0	0.26	77	1.1	2.64
11	9.3	0.33	71	3.3	44.63
12	8.1	0.27	52	3.7	0.00
13	12.9	0.31	51	7.0	59.30
14	4.8	0.16	42	0.0	15.78
15	11.7	0.23	40	0.2	11.39
16	10.5	0.30	37	8.4	3.18
17	12.9	0.56	30	2.9	0.02
18	14.1	0.23	12	8.2	0.26
19	14.4	0.66	5	0.0	0.07
20	12.0	0.14	0	0.0	–
C1	4.5	0.05	33	0.0	–
C2	5.4	0.02	9	0.0	–
C3	7.8	0.02	2	0.0	–

¹The percentage of specific plasma cells in the tissue was calculated as follows: anti-Ag53 + anti-Arg-hgp/Lys-hgp + anti-Arg-pro + anti-Lys-pro. The higher figures of anti-Arg-hgp or anti-Lys-hgp were chosen as the data for anti-Arg-hgp/Lys-hgp.

²The number represents the ΔΔC_t value in real-time PCR analysis, and – indicates that it was undetectable.