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. 2014 Sep 4;33(21):2507–2520. doi: 10.15252/embj.201488398

Figure 5. Dependency of Bu-1a loss on G4 motifs of differing sequence in the +3.5 position.

Figure 5

  1. Bu-1a loss in mutants harbouring different G4 motifs at +3.5 kb from the TSS. (i) rev1 cells with the G4 DNAs removed from both the BU-1A and BU-1B alleles. (ii) Knock-in of the wild-type +3.5 Bu-1 G4 motif. (iii–vi) Knock-ins of G4#1–4. (vii) Knock-in of the ρ-globin intron 2 G4 motif (Sarkies et al, 2010). Red bar = median loss; whiskers = interquartile range; P-values that each distribution is different from control (i) calculated with Fisher's exact test with a bin size of 20%. NS = not significant (P < 0.001).
  2. Pearson correlation of the median level of Bu-1a loss with the in vitro melting temperature of oligonucleotides of each G4 motif.
  3. Pearson correlation of the median level of Bu-1a loss with the maximum non-G loop length of each G4 DNA sequence under test.