Table I.
DSC data for the thermal denaturation of PV2 and mutant proteins
| ΔH(Tm) (kJ mol−1) | Tm (°C) | ΔHvan't Hoff/ΔHcal | ΔTm (°C) | |
|---|---|---|---|---|
| Proteina | ||||
| PV2b | 157 ± 5 | 34.2 ± 0.2 | 0.87 ± 0.19 | – |
| PV2 (2.0M NaCl)b | 357 ± 2 | 64.5 ± 0.1 | 0.84 ± 0.04 | 30.3 |
| Mini-core mutants | ||||
| 1xF(22) | 306 ± 3 | 48.5 ± 0.1 | 0.71 ± 0.01 | 14.3 |
| 1xF(64) | 302 ± 3 | 48.3 ± 0.2 | 0.74 ± 0.02 | 14.1 |
| 1xF(108) | 300 ± 2 | 48.2 ± 0.1 | 0.75 ± 0.01 | 14.0 |
| 2xF(22,108) | 401 ± 2 | 56.9 ± 0.1 | 0.71 ± 0.01 | 22.7 |
| 3xF(22,64,108) | 446 ± 3 | 63.2 ± 0.1 | 0.81 ± 0.02 | 29.0 |
| 3xY(22,64,108) | 437 ± 1 | 67.2 ± 0.1 | 1.12 ± 0.01 | 33.0 |
| 3xW(22,64,108) | 414 ± 3 | 62.0 ± 0.1 | 1.03 ± 0.02 | 27.8 |
| Central core and mini-core mutants | ||||
| 6xFb | 490 ± 3 | 70.7 ± 0.1 | 0.96 ± 0.08 | 36.5 |
| 6xY | 301 ± 1 | 48.9 ± 0.1 | 1.04 ± 0.02 | 14.7 |
| 6xW | 544 ± 1 | 74.6 ± 0.1 | 0.99 ± 0.01 | 40.4 |
a
Buffer contains 0.1M NaCl unless otherwise noted.
b
From Ref.13