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. 2014 Oct 10;24(1):58–69. doi: 10.1002/pro.2586

Figure 1.

Figure 1

Purification and preliminary native gel-based analysis of WzzB and WzzE oligomers. Size-exclusion elution profiles of (a) WzzB and (b) WzzE purified on Superdex-200 column in β-dodecyl-maltoside (β-DDM) as well as the Coomassie-blue stained SDS-polyacrylamide gels demonstrating the purity of each protein after the size-exclusion step; (c) Size-exclusion chromatography of WzzB-β-DDM and WzzE-β-DDM analyzed on Superose-600 column. The SEC trace of standard globular proteins is shown in gray and corresponds to the following molecular masses: 660 kDa—Thyroglobulin, 440 kDa—Apo Ferritin, 158 kDa—Aldolase; (d) Blue native PAGE analysis of the WzzB-β-DDM and WzzE-β-DDM oligomers.