Table II.
Complex | Buffer conditions | Temp | CXCL8 conc. Start/end (μM)c | Final Peptide Conc. (μM) | Molar ratio | FB (%) | Kd (μM)d |
---|---|---|---|---|---|---|---|
WT + h29mera,b | 50 mM Pi, pH 7.0 | 30°C | 90/62 | 711 | 1:11.5 | 87 ± 2 | 103 ± 12 |
WT + h29mera,b | 50 mM Pi, pH 6.0 | 30°C | 100/80 | 850 | 1:10.5 | 98 ± 1 | 17 ± 4 |
WT + h21mera,b | 50 mM Pi, 150 NaCl, pH 6.5 | 25°C | 125/20 | 2660 | 1:134 | 79 ± 3 | 700 ± 90 |
R26C + h29mer | 50 mM Pi, pH 7.0 | 30°C | 150/129 | 1029 | 1:11.2 | 85 ± 3 | 172 ± 24 |
1–66 monomer + h29mer | 50 mM Pi, pH 6.0 | 30°C | 200/135 | 1135 | 1:8.4 | >99 | <5 |
V27P/E29P monomer + h29mer | 50 mM Pi, pH 7.0 | 30°C | 100/65 | 777 | 1:11.9 | >99 | <5 |
V27P/E29P monomer + h21mer | 50 mM Pi, pH 7.0 | 30°C | 100/72 | 461 | 1:6.4 | 90 ± 3 | 43 ± 10 |
V27P/E29P monomer + h21mer | 50 mM Pi, 150NaCl, pH 6.5 | 25°C | 100/69 | 945 | 1:13.8 | 88 ± 2 | 118 ± 14 |
L25Y/V27R monomer + h21mer | 50 mM Pi, pH 7.0 | 30°C | 100/63 | 506 | 1:8 | 77 ± 5 | 135 ± 25 |
L25Y/V27R monomer + h21mer | 50 mM Pi, 150 NaCl, pH 6.5 | 25°C | 150/62 | 1176 | 1:19 | 72 ± 2 | 432 ± 25 |
WT dimer dissociation was coupled to receptor peptide binding
Kd calculated for the dimer.
Concentrations are reported in monomer units.
Kd values are averages calculated from a subset of 8–15 residues; Pi, phosphate.