Figure 1.

Peptide arrays of PDZ domains reveal specificity of D-AKAP2 for PDZK1 and NHERF. Peptide array of PDZ domains overlaid with the D-AKAP2_AKB (a) or with the larger fragment of D-AKAP2 that includes RGS-B (c). Bound AKAP was detected with anti-GST antibody. The corresponding PDZ domains that reacted most strongly with D-AKAP2 are B3: NHERF-1 PDZ1; B6: NHERF-2 PDZ2; F12: PDZK1 PDZ3; and G1: PDZK1 PDZ4. (b) Cartoon model of PDZK1 and NHERF-1 bound to D-AKAP2, which thus serves as an adaptor for coupling D-AKAP2 to receptors or transporters.