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An optimized version (crCD) of the E. coli cytosine deaminase gene codA was introduced into the C. reinhardtii chloroplast under the control of the promoter and 5′ UTR from psaA exon 1. In addition to codon optimization of codA, three amino acid substitutions were made (vertical black lines). Dashed lines show the positions of homologous recombination.
