Figure 5.

Identification of a Q633STOP mutation in the TAA1 gene of UV mutant A1-UV1 by DNA sequencing, and confirmation by restriction digest. Dark grey boxes represent exons. The position of an 820 bp PCR product surrounding the premature stop codon is indicated by a pale grey box. This product was digested with PvuII, which cuts at the sequence CAGCTG; mutation of this sequence causes a change in the restriction pattern relative to the parental A1 strain, as shown by agarose gel electrophoresis (right). The left lane contains GeneRuler DNA Ladder Mix (Thermo Scientific).