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. 2014 Feb 4;77(5):737–747. doi: 10.1111/tpj.12423

Figure 5.

Figure 5

Knock-in of ENODL1 (At5g53870) and knock-down of peroxidases stimulates growth. (a, b) Phenotypes and protein levels of bri1-5 plants and two transgenic lines overexpressing At5g53870 in the bri1-5 background. (a) Comparison of 4-week-old plants exhibiting the partial suppression of bri1-5. (b) Immunoblot analysis showing At5g53870 expression in transgenic lines, compared with the bri105 control. Proteins were extracted from the aerial tissues of adult plants. (c) 35Spro:PRX2 RNAi and 35Spro:PRX73 RNAi seedlings display elongated hypocotyls. (d) Measurement of hypocotyl and root lengths. Seedlings were grown on MS medium for 7 days under long-day conditions (16-h light/8-h dark, with a photon fluence rate of 80 μmol m−2 s−1 at 22°C. Error bars are standard deviations (n = 20; P < 0.001). Different lowercase letters above each bar indicate statistically significant differences. (e) Relative RNA levels of nascent PRX2 and PRX73 in wild-type and transgenic lines expressing PRX RNAi. Error bars are standard deviations (n = 3; P < 0.001).