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. 2014 Sep 17;80(5):809–822. doi: 10.1111/tpj.12671

Figure 7.

Figure 7

Complementation of the Arabidopsis thaliana gntI mutant by expression of chimeric CTS regions fused to the catalytic domain of A. thaliana GnTI. Proteins were extracted from 5–week-old soil-grown plants, separated by SDS-PAGE and complex N–glycans were detected by immunoblotting using antibodies directed against β–1,2-xylose and α–1,3-fucose containing complex N–glycans.(a) Protein extracts from gntI expressing AtNNN-AtGNTI or RRR-AtGNTI.(b) Protein extracts from gntI expressing RNN-AtGNTI, NRR-AtGNTI, RNR-AtGNTI or NNR-AtGNTI. Ponceau S (P.) staining serves as the loading control.