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. 2014 Oct 15;33(23):2765–2781. doi: 10.15252/embj.201488987

Figure 2. Lymphocyte activation and B-cell development are impaired in Malt1 C472A knock-in mice.

Figure 2

  • A, B IL-2 production (A) and proliferation (B) and of naïve T cells stimulated with the indicated concentrations of plate-bound anti-CD3 and anti-CD28 antibodies (pooled from three mice and stimulated in triplicates).
  • C, D Analysis of the presence of peritoneal CD5+IgM+ B1 B cells and splenic marginal zone CD19+CD23loCD21hi B cells in wild-type (+/+), knock-in (ki/ki), or knock-out (ko/ko) mice (n = 3).
  • E Fluorescence staining of spleen sections for the presence of CD21/35+ marginal zone B cells (cyan) and IgD+ follicular B cells (red). Scale bar, 100 μm.
  • F Analysis of total basal immunoglobulin (Ig) levels in the serum of wild-type (+/+), knock-in (ki/ki), or knock-out (ko/ko) mice (n = 8).
  • G, H NP-specific immunoglobulin (Ig) levels in the serum after immunization with NP-ficoll (G) or NP-CGG (H) (n = 8).
Data information: Bars represent means ± SD; differences were statistically significant with P < 0.01 (unpaired t-test) unless indicated otherwise (**P < 0.01; ***P < 0.001; n.s., not significant). Data are representative of four (A, B), three (C–E), or two (F–H) experiments.