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. 2014 Oct 27;33(23):2847–2859. doi: 10.15252/embj.201488889

Figure 4. In vivo analysis of Rad50 mutations in Saccharomyces cerevisiae.

Figure 4

  1. Effects of rad50 mutants on S. cerevisiae survival in the presence of DNA-damaging agents. Plate survival assays show that R1201ScE, S1205ScR, and E1235ScQ mutants are deficient in the DNA damage response, comparable to the Δrad50 strain. K103ScE + R131ScE double mutant shows partially inhibited DNA damage response.
  2. Telomere maintenance assays show altered telomere metabolism for mutations or double mutations at the proposed Rad50 DNA-binding groove (K103ScE, K104ScE, K104ScE + R131ScE, R1201ScE) and the signature motif mutant S1205ScR. A notable exception is the Walker B mutant E1235ScQ, which is not proficient in ATP hydrolysis, suggesting that the ATP-bound, engaged Rad50 dimer is essential for the role of MRN at telomeres.
  3. Summary of S. cerevisiae phenotypic behavior in DNA repair and telomere maintenance compared with the effect of the corresponding tmRad50 mutations on DNA binding in vitro.