Figure 6. HP1γ bridges MSK1 at the chromatin and regulates the expression of specific genes.

1. WT and HP1γ null mouse fibroblasts were stimulated by PMA at the indicated times. Nuclear extract were processed as indicated in Materials and Methods. Western blots were performed with anti-MSK1, anti-HP1γS83p, and anti-histone H3 antibodies.
2. HeLa cells were transfected with siHP1γ or, as a control, siGAPDH and stimulated by PMA (40 min). ChIPs were performed using anti-HP1γ or anti-MSK1 antibodies, or unrelated mouse IgGs as a control. Results are expressed as fold induction versus IgG for each point (**P < 0.01). Enrichment values are the mean of two independent experiments. Significance of the differences was estimated using Student's t-test with a threshold at 5%.

Source data are available online for this figure.