Skip to main content
. 2014 Sep 12;33(22):2606–2622. doi: 10.15252/embj.201489244

Figure 7. HP1γ affects the transcriptional impact of PMA.

Figure 7

MEF-derived cells either HP1γ null or re-complemented with either HP1γ WT or HP1γS83A were either not stimulated or exposed to PMA for 1 h. Total RNA was depleted from ribosomal RNA then sequenced by Next Generation sequencing with a 50+ bp standard.
  1. Venn diagram showing the number of transcripts regulated by PMA stimulation in HP1γ null (blue), HP1γ WT (red), and HP1γS83A (green) MEF-derived cell lines (≥ 1.5-fold change in expression, ≥ 20 reads, P < 0.05).
  2. KEGG functional categories significantly enriched (P < 0.05) for genes differentially expressed in the indicated conditions, depicted according to their P-value [−log10(P-value)].
  3. Fold induction by PMA of a subset of OspF target genes (≥ 2-fold change in expression in HP1γ null) in each of the indicated cell lines. Data and P-values are provided in Supplementary Table S1.