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. 2014 Sep 4;33(22):2659–2675. doi: 10.15252/embj.201489039

Figure 6. Arf1 is involved in mitophagy and autophagy.

Figure 6

  1. arf1 mutants are defective in mitophagy. Strains expressing mt-Rosella were grown at permissive temperature overnight, diluted, grown to logarithmic phase at the indicated temperatures, then shifted to starvation in SD-N for 14 h at indicated temperatures and imaged thereafter. Scale bar, 5 μm. Half of the culture was shifted to restrictive temperature for 1 h and imaged thereafter.
  2. Quantification of data in (A) from three independent experiments in which at least 100 cells were scored. Standard deviation is given.
  3. arf1 mutants are defective in autophagy. Strains expressing GFP-Atg8 from its endogenous promoter were cultured as in (A) and starved in SD-N for 5 h. The arrival of GFP-Atg8 in the vacuole as a readout for autophagy was scored in arf1 mutant cells. Data from three independent experiments in which at least 100 cells were scored are shown. Standard deviation is given.