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. 2014 Oct 8;33(22):2704–2720. doi: 10.15252/embj.201489054

Figure 8. Depletion of SDS22 or I3 interferes with inactivation of Aurora B at centromeres and chromosome segregation during anaphase.

Figure 8

A, B I3 depletion induces SDS22 localization to kinetochores also in anaphase. Stable GFP-SDS22 cells were depleted and processed as in Fig 4C. Scale bar, 10 μm. The percentage of cells with SDS22 on anaphase kinetochores was determined (B). Error bars indicate s.d. of three independent experiments with 20 cells per condition. ***P < 0.001.

C HeLa cells were depleted of indicated proteins and processed for immunodetection of Aurora B or pT232 as in Fig 2A. Note persisting phospho-Aurora B on anaphase chromosomes in SDS22- or I3-depleted cells. Scale bar, 5 μm.

D, E Quantification of signal intensities in (C). Box plot as in Fig 2B. Data from three independent experiments with 50 cells per condition. ***P < 0.001.

F, G Representative images of anaphase cells treated with indicated siRNAs and quantification of cells with anaphase defects. Error bars indicate s.d. of 3 independent experiments with 50 cells per condition. *P < 0.05; **P < 0.01.