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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Fertil Steril. 2014 Nov 5;103(1):270–280.e8. doi: 10.1016/j.fertnstert.2014.09.023

Figure 2. G-CSF treatment improves PLZF+ spermatogonial numbers after busulfan treatment without changing apoptosis.

Figure 2

Testes from mice in Experiment 2 were evaluated on day 8 (24 hours after the last G-CSF/vehicle treatment, see Supplemental Figure 1). (A–D) PLZF+ spermatogonia were quantifed per round seminiferous tubule cross-section from testes of mice obtained from each group. Sections co-stained for (E–H) PLZF and activated Caspase3 or (I–L) PLZF and TUNEL were used to determine the percentage of PLZF+ spermatogonia that were positive for activated Caspase 3 and TUNEL, respectively. The A, B, and C categorical notations above bars denote statistically significant differences between groups (p<0.05) as determined by Student’s T-test. Scale bars = 50µm. The number of round seminiferous tubule cross-sections and cells counted per animal is in Supplemental Table 5.