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. Author manuscript; available in PMC: 2016 Jan 2.
Published in final edited form as: Circ Res. 2014 Sep 23;116(1):35–45. doi: 10.1161/CIRCRESAHA.115.304457

Figure 1. Genome-wide screen to identify genes directly regulated by YAP in cardiomyocytes.

Figure 1

A. Strategy to identify genes directly bound by YAP and differentially expressed in YAP gain- and loss-of-function. ChIP-seq was performed using Flag-tagged YAP in HL1 cells. Tag heatmap shows signal intensity in ChIP and input sample in a 4 kb window centered on YAP peaks. Differential gene expression in YAP loss-of-function and gain-of-function were determined by microarray analysis of E12.5 hearts with cardiomyocyte-restricted YAP knockout, and NRVMs that overexpressed activated YAP. Heatmap shows the row-scaled expression z-score. B. Gene Ontology term analysis of YAP- bound genes. C. Motif analysis of the top 1000 YAP bound regions. D.YAP overexpression induced upregulation of Pik3cb. *, P<0.05.