Figure 4. Lack of NOS1-derived NO underlies the atypical SR Ca2+ leak-temperature pattern in NOS1−/− cardiomyocytes.
(A) Dependence of SR Ca2+ leak on temperature in NOS1−/− cardiomyocytes in the absence or the presence of 1 μmol/L SNAP [at matched SR Ca2+ load ≈75 μmol/L] (* p < 0.05(-)SNAP vs. +SNAP-treated NOS1−/−, Two-way ANOVA). (B) Dependence of SR Ca2+ leak with temperature in WT cardiomyocytes in the absence or the presence of 100 μmol/L L-VNIO [at matched SR Ca2+ load ≈ 75 μmol/L] (* p < 0.05 (-)L-VNIO vs. +L-VNIO-treate WT, Two-way ANOVA).
